老年胃癌和不典型增生患者肿瘤相关抗原表达和DNA含量的研究

Tumor Associated Antigen and Cellular DNA Content in Gastric Cancer and Dysplasia of the Elderly Patients

应用免疫组织化学和自动图像分析(AIAI)技术对128例老年人慢性萎缩性胃炎(CAG)、不典型增生(ATP)、胃癌(GC)及正常胃粘膜进行肿瘤标记物(MG_7)和细胞核DNA含量及形态参数定量测定。结果表明,MG_7的阳性表达率在GC组为88.0％,ATP组8.7％,CAG组3.3％,随病变程度加重,细胞核DNA含量及形态参数均明显增高,DNA含量与MG_7染色程度存在正相关。提示细胞DNA含量、形态参数及MG_7可作为胃癌早期诊断及癌前病变监测的有用指标。

Cellular DNA content, morphologic parameter and expression of tumor associated, antigen(MG_7-Ag) were measured by automatic image analysis instrument (AIAI) and immunohistochemical staining technique respecively in 128 elderly patients with chronic atrophic gastritis, dysplasia and gastric cancer. The results showed that the positive rates of MG_7 staining were 88. 0% in gastric cancer group, 8. 7% in dyaplasia group and 3. 3% in chronic atrophic gastritis group. Cellular DNA content and morphologic patameter were increased as the lesion aggravated. There was a positive correla- tion between cellular DNA content and the degree of MG_7 staining. It suggests that the determination of cellular DNA content, morphologic parameter and MG_7 monoclonal antibody might be helpful in the diagnosis of early gastric cancer and the surveillance of precancerous diseases.