国产因卡磷酸钠对体外破骨细胞性骨吸收的作用

Inhibitory effects of domestic incadronate on osteoclastic bone resorption in vitro

目的 研究国产因卡磷酸钠 (ICD)对破骨细胞性骨吸收的作用。方法 采用破骨细胞与骨片的体外共培养法和显微摄片、显微光密度仪扫描及计算机图像分析技术。结果 ICD(0 5 ,5与 5 0 μmol/L)使体外破骨细胞性骨吸收陷窝数目呈剂量依赖性减少 ,中等浓度 (5 μmol/L)的抑制作用明显高于阳性对照药帕屈磷酸钠 (APD) ,两药均可使吸收陷窝表面积明显降低 ,而且ICD作用更明显。结论 ICD具有较强的直接抑制破骨细胞性骨吸收的作用 。

Objective To study the effects of domestic incadronate(ICD) on osteoclastic bone resorbing lacunae. Methods Rabbits osteoclastes were coincubated with bone slices and microphotographed,microdensitometric scanned and analyzed by computer image analysis system. Results ICD (0 5,5 and 50 μmol/L) caused dose dependent reduction in the number of osteoclastic bone resorbing lacunae. The inhibitory effect ICD (5 μmol/L) was more remarkable than that of aminopropanediphosphonate (APD). Both ICD and APD reduced the surface area of osteoclastic bone resorbing lacunae. The effect of ICD was stronger than that of APD. Conclusion ICD directly suppresses osteoclastic bone resorption and may be effective in osteoporosis.