轮状病毒形态发生时病毒性抗原的免疫胶体金标记定位

ANTIGENIC LOCALIZATION DURING ROTAVIRUS MORPHO GENESIS BY IMMUNE COLLOID GOLD TECHNIQUE

利用免疫胶体金标记技术,对感染轮状病毒(SA11)的细胞样品进行包埋前或包埋后标记,研究病毒抗原的定位,证实了病毒外壳上的主要抗原Vp7非对称地分布在粗面内质网膜胞浆侧的表面;存在于粗面内质网中直径85nm的有包膜毒粒,其外膜上未检测到病毒结构蛋白抗原性,在轮状病毒发生过程中,细胞核周间隙和核外膜起着与粗面内质网相同的作用,结果还显示,直径为50nm的病毒核心仅在胞浆内基质样包含体中形成,病毒颗粒的内壳在其包含体边缘65nm处组装,本文认为,由于轮状病毒复制时是以芽生方式进入粗面内质网池的包膜机制,可能使一部分有包膜的轮状病毒逃脱免疫系统的监视,从而引起体内慢性感染。

Antigenic localization by means of immune colloid gold technique to label the pre-or post-embedding cells infected with rotavirus (SA11) , demonstrated that the VP7, the main antigen in outer capsid of double shelled viron is distributed asymmetrically on the cytoplasmic side of rER membrane. Viral structural proteins are not present,on the surface of enveloped viron (85nm) within rER lumen. The perinuclear space and outer membrane of the nucleus share a common function with rER during the morphogenesis of rotavirus. The results also show that viral cores (50nm ) are formed within the matrix-like inclusion body. The inner capsid of single shelled viroas (65nm)is assembled at the peripheral region of the inclusion. The envelopment mechanism may play an important role in rotavirus immunity in terms of escaping from host defence.