深冷冻治疗肝癌对T淋巴细胞亚群的影响

Effects of cryosurgery on T lymphocyte subgroups in treatment of primary liver cancer

目的探讨深冷冻治疗原发性肝癌对 T 淋巴细胞亚群的影响。方法 61例原发性肝癌,其中深冷冻治疗18例,肿瘤切除27例,剖腹探查16例。于术前、术后2周及术后4周分别从外周血测定 CD3、CD4及 CD8淋巴细胞亚群水平,作统计学处理。结果深冷冻治疗能有效杀死大量的肿瘤细胞,破坏肿瘤对机体免疫功能的抑制作用,术后4周免疫功能明显恢复,与肿瘤切除组无差异(P>0.05)。同时其癌细胞碎片及变性蛋白对机体有免疫刺激作用。而冷冻治疗创伤程度较轻,术后2周对机体 T 细胞免疫功能干扰少,与肿瘤切除组相比有显著性差异(P<0.01)。结论深冷冻治疗疗效明确,手术创伤轻,能明显增强机体的 T 细胞免疫功能。

Objective To investigate the influence of cryosurgery on T lymphocytes subgroups in treatment of primary liver cancer.Methods Of 61 patients with primary liver cancer,18 were trea- ted with cryosurgery,27 with tumor resection and 16 with exploratory laparotomy.Contents of CD3, CD4 and CD8 in peripheral blood in all the patients before and 2 and 4 weeks after the operation were measured.Then statistical analysis was conducted.Results In patients treated with cryosurgery,lots of cancer cells were killed and inhibitory effects of cancer on body's immune function destroyed.The immune function markedly resumed in 4 weeks.But there was no significant difference between these patients with those treated with tumor resection (P>0.05).Meanwhile,chips of cancer cells and de- natured proteins could enhance immunological activities.Compared with tumor resection,cryosurgery was markedly less traumatic to body and of less interference to body's immune function (P<0.01). Conclusion Cryosurgery is less traumatic than tumor resection.In addition,it has definite therapeutic effects on liver cancer and can significantly enhance body's immune function.