原发性胆囊癌端粒酶检测的临床意义

Clinical significance of telomerase detection in primary gallbladder carcinoma

目的探讨端粒酶在原发性胆囊癌中的活性及临床意义。方法采用端粒重复序列扩增法(TRAP)检测了胆囊癌细胞系,46例原发性胆囊癌组织,22例癌旁组织,19例胆囊良性病变。结果胆囊癌细胞系呈端粒酶阳性,癌组织的端粒酶阳性率为84.8%,显著高于癌旁组织和胆囊良性病变(P>0.05)。端粒酶活性与胆囊癌病理类型和分化程度无相关性(P>0.05)。与浸润深度和淋巴结转有显著相关性(P<0.05)。结论端粒酶激活与原发性胆囊癌的发生、发展有密切关系,并有可能成为原发性胆囊癌的临床肿瘤标记物。

Objective To detect the telomerase activity in primary gallbladder carcinoma (PGC) and determine its clinical significance.Methods Telomerase activity was detected by a non-isotopic PCR-based TRAP (telomeric repeat amplication protocol) assay in tissue samples from 46 PGC,22 tumor-adjacent tissues and 19 benign lesions.Results The telomerase positive rate of PGC (84.8%) was markedly higher than those of tumor-adjacent tissues and benign lesions (P<0.01).Telomerase activity was not correlated to pathological types and differentiation of PGC (P>0.05).However,it was significantly correlated with the tumor's deep invasion and lymph node metastasis (P<0.05). Conclusions Telomerase expression might contribute to the progression of PGC and can be used as its tumor marker.