p53、Bcl-2基因调控与细胞凋亡在肾母细胞瘤发生发展中的作用

THE ROLE OF P53, BCL-2 GENE REGULATING FUNCTION AND APOPTOSIS IN ONCOGENESIS OF NEPHROBLASTOMA

目的 分析肾母细胞瘤细胞凋亡和p53、Bcl- 2蛋白表达的相关性及其与肾母细胞瘤发生、组织学类型的关系 ,探讨 p53、Bcl- 2基因调控与细胞凋亡在肾母细胞瘤发生发展中的作用。方法 对 30例肾母细胞瘤用DNA末端标记 (TUNEL)方法检测细胞凋亡 ,免疫组化LSAB法检测p53、Bcl- 2蛋白的表达。结果 30例肾母细胞瘤凋亡指数 (AI)为 1‰～ 40 0‰ ,平均 1 0 0‰ ,p53、Bcl- 2的表达指数PEI、BEI分别为 0～ 30 0‰、1‰～ 360‰ ,平均值分别为 82‰、90‰。统计学分析表明细胞凋亡和 p53与肾母细胞瘤组织学类型有显著相关性 ,细胞凋亡与 p53呈负相关 ,Bcl- 2与组织学类型、细胞凋亡、p53均无关。 结论 p53在调节肾母细胞瘤细胞凋亡中有较为重要的作用 ,p53基因突变可能与机体清除后肾胚胎细胞的凋亡机制丧失 ,生后残存的胚胎组织发生肾母细胞瘤有关。细胞凋亡和 p53突变与肾母细胞瘤的细胞分化、恶性程度有关 ,PEI。

Objective To analyze the correlation between the apoptosis and p53, Bcl-2 expression; the relationship among apoptosis, p53, Bcl-2 expression and the histology type of mephroblastoma, and to investigate the role of p53, Bcl-2 genes in regulating function and apoptosis in the oncogenesis and progression of nephroblastoma. Methods Apoptosis was tested by using TUNEL and p53, Bcl-2 expression was tested by using immunohistochemistry (LSAB) in sections of 30 mephroblastoma paraffin specimens. Results The apoptosis index (AI) of 30 specimens ranged from 1% to 400%, and p53, Bcl-2 expression index (PEI, BEI) were 0～300% and 1～360 respectively. Statistics showed that apoptosis and p53 expression had significant relation with histology type of nephroblastoma, and apoptosis had negative correlation with p53 expression, while Bcl-2 had no relationship with histology type, apoptosis or p53 expression. Conclusion p53 expression may play a more important role than Bcl-2 expression in regulating apoptosis in nephrblastoma. P53 gene mutation may have relation with the loss of the apoptosis by which the body gets rid of rear renal embryonic cells, and the generation of nephroblastoma from embryonic tissue which remained after born. Apoptosis and p53 gene mutation are related with cell differentiation and the aggression of nephroblastoma. PEI and AI tests may provide experimental basis of studying the generation and judging the prognosis of nephroblastoma.