大肠癌患者粪便标本的端粒酶活性研究

Telomerase activity in stool of patients with colorectal cancer

目的 探讨通过粪便途径筛查大肠癌的可行性和新方法。 方法 应用聚合酶链端粒重复扩增 (PCR TRAP)银染技术 ,研究了 43例大肠癌患者粪便中脱落细胞的端粒酶活性表达。结果6 2 8%的大肠癌患者粪便标本中有端粒酶阳性表达。患者粪便标本中端粒酶阳性表达与其大肠癌Dukes分期、淋巴结转移和癌肿部位均未见显著相关 (P >0 0 5 )。 1例结肠腺瘤患者粪便标本端粒酶表达阳性 ,其腺瘤组织也存在端粒酶活性表达。粪便端粒酶检测的敏感性、特异性和阳性预测值分别为 6 2 8%、95 7%和 96 4%。 结论 PCR

Objective To probe into the method for screening colorectal cancer by the way of stool. Methods Telomerase activityin the stool of patients with colorectal cancer was determined by PCR TRAP. Results The positive rate of telomerase activity in the stool of patients with colorectal cancer was 62 8%. Its sensitivity, specifity and positive predictive value were 62 8%, 95 7%, and 96 4%, respectively. Telomerase activity in stool was not obviously correlated with Duke′s stage, lymphatic metastasis and tumor location. Telomerase activity was also found in the stool sample of a patient with colonic adenoma. Conclusion The determination of telomerase activity in the stool of patients with colorectal cancer contributes to the improvement of screening and diagnosis of colorectal cancer.