表皮生长因子受体在前列腺癌雄激素非依赖型细胞系中表达的意义

Epidermal growth factor receptor expression in human prostate androgen unresponsive cancer cells

目的 探讨转化生长因子 (TGF) α和表皮生长因子 (EGF)对前列腺癌雄激素非依赖型细胞系中表皮生长因子受体 (EGFR)表达的调控作用。方法 采用逆转录 多聚酶链式反应和免疫印迹法分别对TGF α和EGF刺激前列腺癌雄激素非依赖型细胞系PC3、ARCaP后EGFRmRNA表达及其蛋白水平进行定量分析。结果 EGF引起PC3、ARCaP的EGFRmRNA升高 ,分别为5.0 1± 0 .45和 2 .41± 0 .2 6,差异无显著性 (P >0 .0 5) ;TGF α引起各细胞系EGFRmRNA升高 ,分别为 9.0 5± 0 .63和 3 .54± 0 .3 3 ,差异无显著性 (P >0 .0 5)。各细胞系EGFR蛋白水平TGF α组明显高于EGF组 (P <0 .0 5)。结论 TGF/EGF EGFR通路在发生发展中起重要作用 ;TGFα EGFR自分泌环在非依赖型前列腺癌中的作用强于EGF EGFR自分泌环。

Objective To elucidate the regulation of epidermal growth factor receptor (EGFR) expression by transforming growth factor (TGF) α and epidermal growth factor (EGF) in human prostate androgen unresponsive cancer cells.Methods EGFR mRNA expression and its protein level were measured by means of RT PCR and Western blot respectively in human prostate cancer androgen unresponsive cell lines, ARCaP and PC3, all treated with exogenous EGF or TGF a.Results In TGFa treated groups the levels of EGFR mRNA were 5.01±0.45 and 2.41±0.26 in PC3 and ARCaP respectively (P>0.05), while in the EGF treated groups the levels of EGFR mRNA were 9.05±0.63 and 3.54±0.33 in them respectively (P>0.05). In all cell lines TGF a induced total EGFR protein levels higher than EGF (P<0.05).Conclusion TGF α/EGF EGFR path way serves as a key growth regulator in prostate cancer.TGF α, but not EGF, would preferentially maintain an autocrine loop in human androgen unresponsive prostate cancer.