肌肉注射内皮抑素基因抑制人大肠癌生长的研究

Growth inhibition of colorectal carcinoma by intramuscular injection of endostatin gene

目的 探讨内皮抑素基因治疗大肠癌简便有效的途径。方法 构建内皮抑素表达载体 ,建立BALB/C nu/n裸鼠大肠癌模型 ,裸鼠分为 3组 ,每组 8只 ,分别通过肌肉注射生理盐水、对照质粒和表达质粒 pST endo ,研究内皮抑素对结肠癌生长的影响。结果 荷瘤小鼠经肌肉注射内皮抑素质粒DNA后 ,肿瘤内细胞凋亡率为 2 4.5± 7.3 ,空白质粒和生理盐水肌肉注射组小鼠肿瘤内细胞凋亡率分别是 8.9± 2 .7和 1 0 .2± 3 .6,前者明显高于后两者 ;肿瘤内微血管密度 (2 1 .7±2 .8)却大大少于空白质粒 (4 4 .6± 1 1 .0 )和生理盐水 (50 .8± 1 3 .4)注射组 ;肿瘤体积在各时间段也明显小于对照的后两组。结论 肌肉介导的内皮抑素基因转移治疗大肠癌效果确切。

Objective To seek an easier and more effective way to treat colorectal carcinoma by endostatin gene.Methods Expression plasmid encoding endostatin was constructed. Animal model for colorectal carcinoma was established.In vivo tumor growth was evaluated by endostatin injection intramuscularly.Results After endostatin plasmid DNA was injected into the mice bearing tumor,tumor cells apotosis rate (24.5±7.3) was obviously higher than that in blank plasmid (8.9±2.7) and saline (10.2± 3.6) injections.However,tumor microvessel density and tumor volume were significantly lower than in the two control groups.Conclusions Endostatin gene transfer by intramuscular injection can treat colorectal carcinoma effectively.