中国南海总合草苔虫抗癌活性成分研究(IV)Bryostatin8,16的分离与结构鉴定

Studies on antineoplastic constituents from marine bryozoan bugula neritina inhabiting south china sea (IV):Isolation and structural elucidation of bryostatins 8 and 16

以体外抗癌活性为指标 ,采用多种层析分离技术 ,从采自广东大鹏湾海域的总合草苔虫中追踪分离得到 8个活性单体。利用电喷雾离子质谱 (ESI- MS)和高分辨 (60 0 MHz)核磁共振二维谱技术测定活性化合物的结构 ,分别鉴定为 :bryostatin 4 ,bryostatin 5 ,bryostatin 6,bryostatin 8,bryostatin 10 ,bryostatin 11,bryostatin16,bryostatin18。其中 ,bryostatin8( )和 bryostatin16( )是首次从南海总合草苔虫中获得 ,并在体外表现出显著的抗癌活性。

Eight active compounds were isolated from Bugula neritina Linnaeus collected off Dapen Bay in Guandong province, guided by P 388 bioassay system by a combination of successive chromatographies and preparative HPLC. By intensive analysis of the data of high resolution 2D NMR spectrum and ESI MS, their structures were elucidated as known bryostatins--bryostatin 4, 5, 6, 8, 10, 11, 16 and 18, among which bryostatin 8 and 16 were for the first time obtained from this animal in South China Sea and they showed significant antineoplastic activities in vitro.