酶解鳀鱼可溶性肽分子组成结构及营养评价

Molecular composition of Enzymolyzed Anchovy Soluble Peptide and its of Nutrition Evaluation

本文叙述了用胃蛋白酶、胰蛋白酶水解的鱼是鱼可溶性肽类水解物 ,经凝胶高效液相色谱和氨基酸分析仪分析测定其肽类分子组成结构和氨基酸组成成分。测得水解产物中肽类相对分子质量在 74 0 0以下 ,其中相对分子质量 660 0～ 74 0 0、由 5 2～ 5 8个氨基酸组成的较长肽链占 1.74 % ;相对分子质量 2 5 0 0～ 5 30 0、由 2 0～ 4 1个氨基酸组成的中长肽链占 2 9.75 % ;相对分子质量在 10 0 0以下的由 2～ 10个氨基酸组成的寡肽占5 0 %。水解物的总氮与氨基酸态氮比为 2 5 .9:1,约有 96%的氨基酸以肽类形式存在 ,4 %为游离氨基酸。测得可溶性肽的总氨基酸含量为 73.98% ,必需氨基酸为 32 .39% ,占总氨基酸的 4 3.78% ,与 FAO/ WHO相比 ,苯丙氨酸为第一限制性氨基酸 ,氨基酸分值为 61。根据分析结果 ,深入探讨了鱼是鱼可溶性肽氨基酸组成的平衡性及其有关寡肽在动物机体的生理功能作用。

The peptides molecular compositions and the amino acids component of the enzymolyzed protein of anchovy soluble peptides hydrolysed by pepsin and trypsin were assayed and determined by gel HPLC and amino acid analytical instrument.The molecular weight of the peptides is less than 7400, of which 1.74 percent is a large peptide chain with composition of 52~58 amino acids and 6600~7400 molecular weight,29.75 percent is a middle large peptide with 20~41 amino acids and 2500~5300 molecular weight , and 50 percent is a oligo-peptide with 2~10 amino acids and less than 1000 molecular weight. The proportion of total nitrogen to rree amino acid nitrogen is 25.9:1 in the hydrolysates. 96 pecent of amino acid exists in form of peptides and 4 percent are free amino acids. The con tent of whole amino acids accounts for 73.98 percent soluble peptides, and the essential amino acid is 32.39 percent or 43.78 percent of the whole amino acids .Comparing whth FAO/WHO , the phenylalanine expresses in the first limited amino acid with the amino acid score of 61. Based on the analysis results, the balance of amino acid composition pf anchovy loluble peptides and the physiological function of loigo-peptide in an animal organism were explored.