斜纹夜蛾核型多角体病毒多角体蛋白基因定位及同源性分析

POLYHEDRIN GENE MAPPING AND GENE HOMOLOGY ANALYSIS OF PRODENIA LITURA NUCLEAR POLYHEDROSIS VIRUS

斜纹夜蛾核型多角体病毒(Prodenia litura Nuclear Polyhedrosis ViruS,PINPV)属杆状病毒A亚群,其宿主昆虫对农业生产危害极大,因此详细研究这种病毒不仅具有理论意义,而且在杀虫剂的研究方面亦有一定的实际意义。然而过去对这种病毒的研究较少,尤其是在分子生物学特性方面,而对其基因结构的研究目前尚未见报道。本文报道P1NPV多角体蛋白基因在基因组上的定位。根据核型多角体病毒多角体蛋白基因高保守性的特点。

To map the polyhedrin gene of the PlNPV, AcNPV -DNA EcoR I -I fragment was used as a probe to hybridize with the P1NPV-DNA restriction fragments immobolized on nitrocellulose filte under stringent and nonstringent conditions. Under stringent condition (68℃),no hybridization was found between the probe and the P1NPV-DNA. But under nonstringent Condition (35℃, 20% formamide), the P1NPV-DNA EcORI-6.8kb fragment can hybridize with the probe. By using a range of formamide. concentration to vary the hybridization conditions, the Tm value (71℃), mismatch rate(l8%),and polyhedrin gene homology(82%)of the P1NPV-AcNPV DNA hybrid was evaluated.