大肠腺瘤核仁组成区嗜银蛋白的定量研究

A Quantitive Study of Argyrophilic Proteins of Nucleolar Organizer Regions in Colorectal Adenomas

用核仁组成区嗜银蛋白（ＡｇＮＯＲ）的银染技术研究了５７例经内镜切除的大肠腺瘤的ＡｇＮＯＲ计数变化，以探讨ＡｇＮＯＲ计数评价大肠腺瘤增殖性的价值。结果显示，管状绒毛状大肠腺瘤的ＡｇＮＯＲ计数明显高于管状腺瘤，而又明显低于绒毛状腺月（Ｐ＜０．０５）；随着腺瘤异型增生的出现和程度的加重，其ＡｇＮＯＲ计数逐步增加（Ｐ＜０．０５）；随着腺瘤直径的增大，其ＡｇＮＯＲ计数亦逐渐递增（Ｐ＜０．０１），且腺瘤的ＡｇＮＯＲ计数与其直径呈现有意义的正相关（ｒ＝０．３８，Ｐ＜０．０２５）；腺瘤的数目对ＡｇＮＯＲ计数无明显影响；位于左半结肠的腺瘤的ＡｇＮＯＲ计数高于位于右半结肠的腺瘤（Ｐ＜０．０５）。结果提示ＡｇＮＯＲ计数反映了大肠腺瘤的增殖活性，它可作为评价大肠粘膜异型增生的定量参数。

Using argyrophilic proteins of nucleolar organizer regions (AgNOR)silver-staining tech-nique, the AgNOR counts in fifty seven specimens of colorectal adenomas (CA)were exam-ined to investigate their value in assessing the proliferative activity of CA. The AgNORcounts of tubular-villous CA were significantly higher than those in tubular CA, but lowerthan those in villous CA(p<0.05),and increased with the appearance and progression ofdysplasia in CA(p<0.05),as well as with the increase in the diameter of CA(p<0.01)。The AgNOR counts were positively correlated to the diameter of CA (r=0.38, p<0. 025).But the number of CA bore no influence on its AgNOR counts, In addition, the AgNORcounts in the left-sided CA were markedly higher than those in the right-sided CA(P<0.05). These findings suggested that the AgNOR counts may reflect the prolifelative activityof CA. Thus it may be used as a quantitative parameter for the assessment of colorectal mu-cosal dysplasia.