肾衰冲剂对大鼠残余肾系膜细胞重塑的影响

Effect of SSCJ on Remodeling Mesangial Cells in Rats Remnant Kidney

目的 :研究 5 / 6肾切除大鼠残余肾系膜细胞重塑时α SMA的变化 ,进一步探讨肾衰冲剂治疗“慢肾衰”的作用机制。方法 :应用免疫组化技术检测大鼠残余肾系膜细胞内α SMA的表达情况和细胞外基质 (ECM )的变化。结果 :残余肾未硬化区肾小球系膜细胞内α SMA表达明显增强 (2 45± 0 36 ) ,硬化区肾小球α SMA表达降低(0 37± 0 0 8) ,经肾衰冲剂治疗后增高的α SMA表达下调 (1 92± 0 31) ,细胞外基质fibronectin、collagen Ⅳ含量减少 ,肾小球硬化指数下降 (2 5 7± 0 35→ 1 6 3± 0 2 4)。结论 :α SMA可作为系膜细胞重塑的标志 。

Objective: To study α SMA expression of remodeling mesangial cells,and clarify the mechanism by which the adminstration of ShenShuaiCongJI(SSCJ) in remnant kidney of 5/6 nephrectomized(SNX) rats. Methods: This experiment included three groups:Sham group,Control group and SSCJ group.the following parameters were studies:the immunohistochemical staining for α SMA,fibronectin(Fn),collagen Ⅳ(Col Ⅳ);the glomerulus sclerosis index(GS index). Result :α SMA was overexpressed in unsclerotic glomerulus of remnant kidney(2 45±0 36) and decreased in sclerotic glomerulus(0 37±0 08).SSCJ could decrease α SMA expression (1 92±0 31) and GS index(2 57±0 35→1 63±0 24). Conclusion: α SMA should be a marker of remodeling mesangial cells.SSCJ could inhibit α SMA overexpression,furthermore,retard progression of remnant kidney scarring.