摘要
目的 观察脑缺血再灌注后白细胞介素 1受体拮抗剂 (IL 1Ra)mRNA的表达。②方法 采用线栓法制备SD大鼠局灶性大脑中动脉阻塞 (MCAO)动物模型 ,应用RT PCR方法观察脑缺血再灌注对大鼠IL 1RamRNA表达的影响。③结果 MCAO模型大鼠缺血侧大脑皮质再灌注后 12 ,2 4h的IL 1RamRNA表达与正常对照组比较明显增加 (F =39.84,q=9.0 3,15 .91,P <0 .0 0 1) ;缺血侧纹状体缺血再灌注后 12h的IL 1RamRNA表达与正常对照组比较明显增加 (F =7.37,q=6 .5 4,P <0 .0 0 1)。④结论 脑缺血后内源性IL 1RamRNA表达增加 ,可能是机体对缺血性损伤的自我保护机制。
Objective\ To investigate the expression of interleukin 1 receptor antagonist (IL 1Ra) mRNA after cerebral ischemia reperfusion in rats. \ Methods\ RT PCR was used to explore IL 1Ra mRNA expression in middle cerebral artery occlusion (MCAO) rat model. \ Results\ IL 1Ra mRNA expression was rapidly induced after 12 and 24 hours of MCAO reperfusion as compared with the normal control group( F=39.84, q=9.03, 15.91,P <0.001). The expression of IL 1Ra mRNA after 12 hours of reperfusion in ischemic cortex increased markedly compared with the normal control( F=7.34, q=6.54, P <0.001).\ Conclusion\ Our results indicated that IL 1Ra mRNA may play an important role in the protection of ischemic brain damage.
出处
《青岛大学医学院学报》
CAS
2001年第4期271-273,共3页
Acta Academiae Medicinae Qingdao Universitatis
基金
国家自然基金重点资助项目 ( 39730 5 10 )
