肝细胞癌及相关慢性肝病的乙型肝炎病毒检测

Test of Hepatitis B Virus in Hepatocellular Carcinoma and in the Related Chronic Liver Diseases

为了探讨乙型肝炎病毒 (HBV)的致癌机理 ,采用免疫组化染色和原位分子杂交方法 ,对慢性肝炎、肝硬化、癌旁肝硬化、肝细胞癌和正常肝组织共 10 8例进行乙型肝炎表面抗原 (HBsAg)、核心抗原 (HBcAg)和HB VDNA检测 .结果 :HBsAg ,HBcAg和HBVDNA的阳性率分别为慢性肝炎 61 9% ( 13 /2 1) ,4 2 9% ( 9/2 1)和75 0 % ( 12 /16) ;肝硬化 64 0 % ( 16/2 5 ) ,3 6 0 % ( 9/2 5 )和 83 3 % ( 15 /18) ;癌旁肝硬化 72 7% ( 16/2 2 ) ,61 1%( 11/18)和 85 7% ( 12 /14 ) ;肝细胞癌 4 5 2 % ( 14 /3 1) ,5 0 0 % ( 14 /2 8)和 64 3 % ( 9/14 ) ;正常肝组织不表达 .慢性肝炎、肝硬化和癌旁肝硬化HBVDNA阳性信号较肝细胞癌多而强 .结果表明大多数肝细胞癌与HBV感染所致的慢性肝炎和肝硬化密切相关 ,癌旁肝硬化可能为癌前肝硬化在癌周的残留 .

In order to study the carcinogenesis machenism of hepatitis B virus (HBV), HBsAg, HBcAg and HBVDNA were determined by immunohistochemical staining and molecular hybridization in 108 patients with chronic hepatitis, liver cirrhosis, paratumor cirrhosis, hepatocellular carcinoma (HCC) and normal liver tissues. The positive rates of HBsAg, HBcAg and HBVDNA were 61 9% (13/21), 42 9% (9 21) and 75 0% (12/16) in chronic hepatitis; 64 0% (16/25), 36 0% (9/25) and 83 3% (15/18) in liver cirrhosis; 72 7% (11/18), 61 1% (11/18) and 85 7% (12/14) in paratumor cirrhosis, as well as 45 0% (14/31), 50 0% (14/28) and 64 3% (9/14) in HCC The normal liver tissues were negative. There were stronger HBVDNA granules in chronic hepatitis, liver cirrhosis, paratumor cirrhosis than those in HCC. The results showed that most of the HCC cases were closely associated with chronic hepatitis and liver cirrhosis of the infection of HBV. The paratumor cirrhosis may be a sequential lesion of precancerous cirrhosis around HCC.