尿中血管内皮生长因子表达与膀胱移行细胞癌诊断及术后监测

Urinary Vascular Endothelial Growth Factor and Its Correlation with the Diagnosis and Prognosis of Bladder Transitional Cell Carcinoma

目的：探讨尿中血管内皮生长因子（VEGF）表达与膀胱移行细胞癌的关系，以及尿VEGF做为瘤标的价值。方法：用抗体夹心ELISA法测量58例研究对象的尿VEGF，并与尿细胞学检查比较（双盲法）。其中28例为膀胱移行细胞癌，每3个月随访1次，6例复发。结果：尿VEGF在膀胱癌组高于非膀胱癌组（P＜0．001）；G1级癌分别低于G2、G3级癌（P＜0．05），在早期复发组高于未复发组（术前）（P＜0．05），尿VEGF诊断实验的特异度和灵敏度分别为93．3％、85．7％。结论：尿VEGF水平可反映膀胱癌的预后、复发；尿VEGF做为诊断和术后监测瘤标有较高价值，优于尿细胞学检查。

Objective Urinary vascular endothelial growth factor (VEGF) and its correlastion with bladder transitional cell carcinoma (BTCC) was studied and its value to be a tumor marker for detection and postoperative surveillance of BTCC was assessed． Methods VEGF was measured by quantitative sandwich enzyme－linked immunosorbent assay (ELISA) in the urine of a total of 58 study subjects.and was compared directly with the cytologic effect (Double－blind method). Twenty－two cases of BTCC had postoperative surveillance every 3 months.and 6 cases relapsed． Results The urinary VEGF was higher in the patients with BTCC than the others without BTCC (P＜0．001)． There was significance between G1 and G2 (P＜0．001) or G1 and G3 (P＜0．001)． The mean urinary VEGF expression in the recurred patients was higher than that of unrecurred patients (P＜0．05)． The sensitivity and specificity of urinary VEGF for diagnosing BTCC were 85．7％,93.3％ respectively． Conclusion VEGF is higher in the urine of patients with BTCC and is correlated with the prognosis and recurrence of BTCC． As a tumor marker for detection and surveillance of BTCC.urinary VEGF is of greater value than cytology．