肝癌^(188)Re放射免疫治疗的实验研究

Experimental Study on 188Re－Radioimmunotherapy for Hepatocellular Carcinoma

目的：研究新型核素188Re标记的抗人肝癌单抗片段HAb18 F（ab′）2对荷肝癌移植瘤裸鼠的抑瘤效应。方法：建立荷人肝癌移植瘤裸鼠模型。选择肿瘤体积为（30～150）mm3的动物随机分5组，其中3组分别尾静脉注射188Re－HAb18 F（ab′）2 3．7、11．1和18．5MBq，给药2次，观察动物体重和肿瘤体积变化。处死动物，摘取瘤体，石蜡包埋，进行HE染色。结果：3组剂量均有不同程度的抑瘤作用，且随着剂量的增加，抑瘤效应增加。低剂量组和中剂量组动物平均体重和生理盐水对照组相比无显著性差异(P＞0．05)。组织学检查表明，随着剂量的增加，肿瘤细胞核形态发生明显的变化，从凝固、核碎裂直至溶解。结论：188Re－HAb18 F（ab′）2是一种新型核素的肝癌导向治疗剂，具有潜在的临床应用前景，本文的研究可为其过渡到临床研究提供参考依据。

Objective To investigate the inhibition of tumor growth of 188Re－labeled hepatoma McAb fragment against xenografts in nude mice． Methods Nude mice bearing human hepatocellular carcinoma with volume of 30～150mm3 were divided into 5 groups randomly． Three groups of them were injected twice with 3．7,11．1 and 18．5 MBq of 188Re－HAb18 F(ab′)2 via tail vein to observe the changes of animal weight and tumor size． After the animals were killed.the tumors were excised and embedded in paraffin.then stained with hematoxyclin and eosinHE． Results The tumor of mice which received three different conjugate doses were suppressed in some extent.in which the high dose group resulted in a remarkable inhibition． The weight of animals treated with low dose and median dose showed no significant discrepancy versus the control group (P＞0．05)． After increasing of dose.HE staining showed that the tumor cell nuclei were pyknotic.karorrhectic and autolytic． Conclusion 188Re－HAb18F(ab′)2 is a new type targeting drug for the treatment of hepatocellular carcinoma and prospects to be tested in clinic． The studies of this paper can provide a reference for clinical trial．