高效液相色谱法研究尼莫地平包合物水溶液的稳定性

Studies on Photochemical Kinetics of Nimodipine and Its β-CD Inclusion Complex in Water Solution by HPLC

目的 :建立高效液相色谱法分离尼莫地平及其光解产物的方法 ,并用以比较研究尼莫地平及其 β-环糊精包合物水溶液的光解稳定性。方法 :光照尼莫地平及其β-环糊精包合物水溶液 ,间隔一定时间取样。以 C1 8反相柱 ,甲醇 -水 ( 33∶ 6 7)为流动相 ,在 2 38nm处 ,用高效液相色谱法分离并测定尼莫地平含量。结果 :在选定的条件下 ,尼莫地平及其光解产物得到分离。外标法测定了尼莫地平含量。结论 :方法简便 ,准确。尼莫地平及其包合物水溶液的光解符合一级动力学方程 。

AIM The purpose is to establish a reversed phase high performance liquid chromatographic Method for the separation of nimodipine and its UV photodecomposition product and comparatively study on photochemical kinetics of nimodipine and its β CD inclusion complex. METHODS A RP HPLC was used on C 18 column with a mobile phase of methanol water(33∶67) for separation of nimodipine and its UV photodecomposition product and the detection wavelength at 238nm. RESULTS A HPLC method was established for the determination of nimodipine and was used to study the kinetics of nimodipine and its β CD inclusion complex in water solution. CONCLUSION The method is convenient, accurate and precise. The behavior of UV photodecomposition of nimodipine and its β CD inclusion complex in water solution followed first order reaction. Half time of nimodipine in β CD inclusion complex was increased.