肾宝合剂中淫羊藿苷含量测定方法的改进

Improvement of Determination of Icariin in Shenbao Mixture

目的 :测定肾宝合剂中淫羊藿苷的方法改进。方法 :采用 HPLC法 ,以 Dikma Diamonsil ODS( 2 5 0× 4 .6 mm,5 μm)为色谱柱 ,乙腈 -水 -冰醋酸 ( 30∶ 70∶ 1 )为流动相 ,流速 1 ml/ min,检测波长 2 70 nm。结果 :淫羊藿苷的平均回收率为 97.79% ,重复性试验的 RSD为 2 .1 1 % ,符合分析要求。结论 :采用 HPLC法测定比汇仁制药标准用的 TLC法简便、准确 。

AIM The purpose is to develop the analysis method of Icariin in Shenbao mixture; METHODS A HPLC method was adopted .The measurement condition used are as follows: Dikma Diamonsil ODS column; mobile phase: acetonitrile water acetic acid glacial(30∶70∶1); flow rate 1.0ml/min; wavelength for measure 270 nm; The conditions of extraction and determination were optimized. RESULT The relative standard deviation of the reproducibility was 2.11% and the average recovery rate was 97.79%. CONCLUSION The method was suitable for qualitative analysis of icariin in Shenbao mixture.