人绒毛膜促性腺激素新的制备方法的研究

A New Preparation Method of Human Chorionic Gonadotropin

目的 :提高人绒毛膜促性腺激素 ( HCG)的活性回收率 ,简化 HCG的纯化步骤。方法 :p H 4 .0 ,0 .0 5mol/L HOAc-Na Ac缓冲液提取 ,人造沸石吸附和 DEAE-纤维素层析法。结果 :该法可将 HCG粗品 ( 1 2 IU/mg)纯化到 32 0 0～ 36 0 0 IU/mg,活性回收率在 70 %至 80

AIM To improve the purification method of Human Chorionic Gonadotropin (HCG). METHODS Extracted with 0.05 mol/L HOAc NaAc buffer (pH 4.0) and then purified by permutit absorption or chromatography on DEAE cellulose. RESULTS and CONCLUSION HCG can purified up to 3500 IU/mg from rough HCG (12 IU/mg). The recovery of biological activity is 70%~80%.