低能量氦氖激光对周围神经再生的电生理研究

Electrophysiological study of low energy He-Ne laser on regeneration of peripheral nerve

目的 探讨低能量氦－氖激光对周围神经再生的影响。方法 用 ４４ 只体质量 ２．５ｋｇ左右的家兔随机分为４，８，１２及１６周４个观察组，照射组各用兔６只，对照组各用５只，麻醉后，均切断左侧腓总神经，用９／０尼龙单丝对端吻合神经外膜。照射组在术后１ｄ开始用８３１３－Ｂ型低能量氦－氖激光仪经皮肤照射 Ｌ５，６脊髓节段，每天照射 １５ ｍｉｎ，共照射 ４ｄ． 对照组不照射，均按期观察．结果 术后 ４周，可在照射组看到细小而稀少的再生轴突，对照组直到术后８周才能看到（Ｐ＜０．０１）．照射组的腓总神经潜伏速率均优于对照组（Ｐ＜０．０１），动作电位波幅照射组也优于对照组．胫前肌肌纤维横纹，照射组１６周时很清楚，对照组不甚清楚 展趾功能到术后１６周时，照射组与健侧相同，对照组才恢复到照射组１２周的水平．结论 低能量氦－氖激光促进了脊髓运动神经细胞功能，加速了轴突再生．

Objective This experiment was to elucidate the influence of the low energy He-Ne laser on the function of regeneration of peripheral nerve. Methods Forty-four rabbits about 2.5 kg body weight were used in the experiment. The animal were divided into 4, 8, 12, 16 week groups according to the observation period. Six animals were used in each irradiated group and in the control group 5 rabbits were used in each observation period. The regeneration of the axon and myelin sheath, the latent rate of the common peroneal nerve, the conditions of the anterior tibital muscle and the toe expansion test were all observed systematically in both groups. Results A few thin regenerated axon was seen at 4 week in the irraditated group, while in the control group it might he seen at 8 week ( P <0. 01). A low amplitude latent rate of the common peroneal nerve is determined at the peroneal side of the anterior tibial muscle in a few animals at 4 week of the irradiated group, and it is not observed in the control group, from 12 to 16 week. The latent rate of the common peroneal nerve was the irradiated group than in the controlled ( P <0.01 ).The amplitude of the irradiated group was higher than in the controlled. The regeneration of the myelin sheath was evident in the irradiated group, and also the section of the muscle fibers anterior tibial was more visible than the controlled. 16 weeks postoperatively, the toe extension test was normal in the irradiated group, while in the control group it was the sante as seen at 12 week after operation in the irradiated group. Conclusion Now it was certain that the low energy He-Ne laser could promote the function of the spine motor nerve cells and accelerate the axonal regeneration.