HPLC法测定咳喘平中橙皮苷的含量

Determination of hesperidin in the kechuanpin by HPLC

目的 :测定咳喘平中橙皮苷的含量。方法 :高效液相色谱法 ,Nona- Pak C1 8柱 ,乙腈 -水 -磷酸 ( 2 1∶ 79∶ 0 .0 1)为流动相 ;检测波长 2 84 nm。结果 :平均回收率为 96.6% ,n=5 ,RSD为 2 .8% ,线性范围为 0 .15 872～ 0 .793 6ug,相关系数为 0 .9999。结论 :该方法简便、准确。

OBJECTIVE:To determinate the quantity of hesperidin in kechuanpin.METHOD:A colum of Nona Pak C 18 was used.The mobile phase was CH 3CN-H 2O-H 3PO 4(21∶79∶0.01). The detection wavelength was 284nm.RESULTS:The average recovery was 96.6%(n=5),RSD=2.8%,The linear range was 0.15872～0.7936ug (r=0.9999).CONCLUSION:The method was simple,rapid and sensitive.