肝细胞癌热休克蛋白70过表达与自发性癌细胞凋亡

Overexpressiou of heat shock protein 70 and spontaneous cancer cell apoptosis in hepatocellular carcinoma

目的 探讨肝细胞癌（ｈｅｐａｔｏｃｅｌｌｕｌａｒ ｃａｒｃｉｎｏｍａ， ＨＣＣ）热休克蛋白７０（ｈｅａｔ ｓｈｏｃｋ ｐｒｏｔｅｉｎｓ ７０，ＨＳＰ７０）的表达及其与自发性癌细胞凋亡的关系。方法 采用免疫组化ＳＰ法和原位末端转移酶标记法（ＴＵＮＥＬ）检测６８例ＨＣＣ手术标本中ＨＳＰ７０表达和细胞凋亡指数，以癌旁肝组织作为对照。结果 ＨＣＣ和癌旁组织中ＨＳＰ７０阳性率分别为７１％和２８％，过表达分别为５３％和２１％，两组之间差异有非常显著意义（Ｐ＜０．０１），ＨＳＰ７０阳性率和凋亡指数有正相关关系（ｒ＝０．８７６ ５，Ｐ＜０．０５），两者都随ＨＣＣ组织学分级增高而增加。结论 ＨＣＣ组织中存在ＨＳＰ７０过表达和自发性癌细胞凋亡，两者正相关性机理尚不明了，可能是ＨＣＣ生物学恶性行为的标志。

Objective: To evaluate the expression of heat shock protein 70 (HSP70) and its relation to spontaneous cancer cell apoptosis in hepatocellular carcinoma (HCC). Methods A series of 68 resected HCC tissues from untreated patients and 68 adjacent normal liver tissues were analyzed for HSP70 expression and apoptosis by immunohistochemical SP and TUNEL methods. Results HSP70 was detected positive in 48/68(71%) cases of HCC and 19/68(28%) cases of the adjacent liver tissues. Overexpression of HSP70 was found in 36/68(53%) cases of HCC and 14/68(21%) cases of the liver tissues. The positive rate and overexpression of HSP70 were significantly higher in HCC than in the liver tissues (P<0.01). Apoptosis index was 8.9 in HCC and 1 .9 in the liver tissues, showing significant different between the two groups (P<0.01). The positive rate of HSP70 was correlated with apoptosis index (r=0.8765, P<0.05) and both of them correlated with HCC histological grades. Conclusions These results suggest that there is an overexpression of HSP70 and spontaneous cancer cell apoptosis in HCC. They seem to be markers of poor proliferation.