经输血传播病毒在肝外组织中的检测及其意义

Significance of detection of transfusion transmitted virus in extrahepatic tissues

目的 探讨经输血传播病毒（ＴＴＶ）在肝及肝外组织的定位、分布及其意义。方法 采用ＰＣＲ及原位杂交方祛，对１３例非甲－非庚型病毒性肝炎患者的尸解肝、胰、肾、脾、睾丸和心脏石蜡包埋组织中的ＴＴＶＤＮＡ进行了检测。结果 ５例肝组织、３例肾脏及胰腺、脾脏组织各２例中检出了ＴＴＶ核酸杂交信号，２例睾丸及心肌组织各检出１例。阳性信号主要定位于肝及肝外组织实质细胞的核内。这些肝外组织未见明显的病理损伤改变。ＰＣＲ的检出率与原位杂交基本一致。结论 ＴＴ病毒能感染肝及肝外组织，肝外组织中的感染可能与ＴＴ病毒的再度感染及在不同人群中的较高感染率有关。

Objective: To investigate the location and distribution of transfusion transmitted virus (TTV) in the liver and extrahepatic tissues. Methods TTV DNA was detected in paraffin-embedded autopsy liver and extrahepatic tissues, including liver, pancreas, kidney, spleen, testicle, and heart from 13 patients with non A-G hepatitis by nested-PCR and in situ hybridization (ISH) techniques. Results Positive hybridization signals of TTV nucleus acid were observed in 5 liver, 3 kidney, 2 pancreas and spleen tissues, respectively. TTV DNA was detected in one from each two samples of testicle and heart. Positive signal was mainly located in nucleon of the liver and extrahepatic parenchymal cells. There was not obvious pathological damage in those extrahepatic tissues. The positive rate of PCR approximately corresponded to ISH. Conclusions TTV can infect hepatic and extrahepatic tissues and its infection in extrahepatic tissues aught be responsible for a state of reinfection and higher prevalence in different population.