乙型肝炎DNA疫苗诱导小鼠免疫反应的实验研究

Immune response induced by hepatitis B DNA vaccine pCI-S-IRES- ProT α

目的 探讨ＤＮＡ疫苗ｐＣＩ－Ｓ－ＩＲＥＳ－ＰｒｏＴ α接种小鼠后的免疫反应变化。方法用基因重组技术构建ＤＮＡ疫苗，脂质体介导转染ＮＩＨ－３Ｔ３细胞，并给小鼠进行ＤＮＡ疫苗接种。ＥＬＩＳＡ检测ＨＢｓＡｇ、抗－ＨＢｓ，ＲＴ－ＰＣＲ检测ｐＣＩ－Ｓ－ＩＲＥＳ－ＰｒｏＴ α的表达。结果 ｐＣＩ－Ｓ－ＩＲＥＳ－ ＰｒｏＴ α、ｐＣＩ－Ｓ在细胞中高效表达， ｐＣＩ－Ｓ－ＩＲＥＳ－ＰｒｏＴ α接种小鼠产生的体液反应和特异Ｔ淋巴细胞增值反应强于ｐＣＩ－Ｓ组。结论 ＰｒｏＴ α可增强接种小鼠的免疫反应。

Objective: To investigate immune response in mice inoculated with DNA vaccine. Methods pCI-S and pCI-S-IRES-ProTα were constructed by gene technology, transferred into cell line NIH-3T3 mediated by lipofectamine. Mice were inoculated with these plasmids too. HBsAg and HBsAb were detected by ELISA, and transcriptions of these plasmids were detected by RT-PCR. Results pCI-S-IRES-ProTα, and pCI-S were effectively expressed in cultured cells and vaccinated animals. Humoral immune and specific T-cell proliferative responses were stronger in pCI- SIRES group than pCL-S group. Conclusions Plasmids coexpression of prothymosin α and hepatitis B surface antigen can improve immune responses significantly.