肝素对深Ⅱ度烧伤大鼠的影响

The effect of heparin on the deep second-degree burn in scalded rats

目的 探讨肝素对深Ⅱ度烧伤大鼠的治疗效果。 方法 将 30只SD大鼠随机分为两组 ,造成 2 0 %TBSA深Ⅱ度烧伤后 ,分别接受肝素 (10 0U·kg-1·d-1)及等渗盐水皮下注射 ,1次 /d ,观察创面愈合情况并进行免疫学检测。 结果 实验组创面愈合所需时间明显少于对照组 ,两组间差异有显著性意义 (P <0 0 0 5 ) ,巨噬细胞吞噬功能明显增强 (P <0 0 5 )。 结论 肝素皮下注射能加速创面愈合 ,并可增强机体细胞免疫水平及巨噬细胞吞噬能力 ,但对体液免疫无影响。

Objective To investigate the effects of heparin on deep second degree burn in scalded rats. Methods Thirty Sprague Dawley rats inflicted with 20% TBSA deep second degree scalding on the back were employed as the model and were randomly divided into two groups.i,e heparin(H) and normal saline groups(N).Heparin (100U·kg -1 ·day -1 ) was injected subcutaneously to the rats in H group while normal saline to those in N group once a day.Wound healing rate and immunological indices were determined simultaneously. Results (1)The wound healing time was much shorter in H group than that in N group (P<0 005).The granulation tissue growth condition examined by LM and EM was much better in H group that than in N group.(2)The lymphocytic transforming ratio in H group was evidently increased (P<0 005).And the phagocytic function of the macrophages increased significantly(P<0 05).But there was no difference between the two groups in terms of hemoltytic plaque test (P>0.05). Conclusion Injection of heparin subcutaneously could accelerate burn wound healing and strengthen the systemic cellular immune potential and the macrophage phagocytic capacity of the host,but exerted no effect on the humoral immunity. [