少突胶质细胞与神经元相互作用时神经元细胞内钙离子浓度的变化

Change of calcium concentration in neurons treated with oligodendrocyte

目的 研究少突胶质细胞与神经元相互作用时神经元细胞内钙离子浓度的变化。方法 用分离培养的少突胶质细胞及其细胞碎片分别作用于培养的PC12细胞 ,于不同时相点在观察PC12细胞突起变化 ,同时检测PC12细胞内钙离子浓度。结果 对照组有少量4 5Ca通过细胞膜流入PC12细胞内 ,当少突胶质细胞及其细胞碎片加入后很快就出现细胞内钙显著增高 ,但在加入 4h后 ,少突胶质细胞及其碎片处理后其细胞内钙较处理 10min有显著下降 ,但即使在处理后 12h仍与正常对照组有显著性差异 (P <0 .0 5 )。钙通道阻滞剂尼莫通对少突胶质细胞引起的细胞内钙增加无明显的抑制作用 ,且尼莫通亦不能阻止少突胶质细胞引起的PC12细胞突起回缩及塌陷。结论 少突胶质细胞作用于PC12细胞时引起的PC12细胞内钙离子浓度的增加可能与突起的塌陷有关。

Objective To investigate the change of calcium concentration in neurons treated with oligodendrocyte. Methods The cultured PC12 cell was treated with oligodendrocyte and its debris respectively, and intracellular concentration of calcium of PC12 cell was detected at different time after treatment. Results Only a small amount of 45 Ca entered PC12 cells in control group, and it showed a rapid increase just as the oligodendrocyte or its debris was added, but decrease significantly 4 h after treatment when compared with that of 10 min and up to 12 h it remained a significantly higher level than that of 10 min after treatment ( P <0.05). Furthermore, Nimotop, an inhibitor of calcium channel, could neither inhibit the increasing of calcium in PC12 cells nor the neurite collapse caused by oligodendrocyte. Conclusion The increase of calcium in PC12 cells caused by oligodendrocyte may be involved in the neurite collapse of PC12 cells.