肺癌K-ras基因突变与呼吸道合胞病毒感染关系的研究

Study on the relationship between K-ras gene mutation and respiratory syncytial virus infection in lung cancer

目的 探讨肺癌K ras基因突变与呼吸道合胞病毒 (RSV)感染的关系。方法 应用反转录聚合酶链反应 (RT PCR)结合单链构象多态性分析 (SSCP)免疫组化和ELISA方法检测肺癌组织中RSV、K ras基因和血清sIgM抗体。结果 ①RT PCR显示17.9% ( 7/3 9)的肺癌标本中存在有RSV基因序列。②RSVRT PCR阳性的肺癌组织冰冻切片免疫组化显示RSV抗原在细胞浆呈弥漫性着色。③不经反转录反应直接作PCR扩增 ,则RSV阳性的标本不能观察到RSV特异的电泳区带。④SSCP电泳示 2 5 .6% ( 10 /3 9)的肺癌组织标本中K ras基因的第 12位密码子有突变。⑤肺癌组织RSV基因的检出与K ras基因突变结果经配对计数资料McNemar检验分析 ,χ2 =0 .3 6,P >0 .0 5。结论 肺癌组织内有RSV感染 ,可能和K ras基因的突变有关。

Objective To explore the relationship between respiratory syncytial virus (RSV) infection and K ras gene mutation in lung cancer. Methods The RSV and K ras gene in lung cancer and specific IgM in serum were detected by reverse transcription PCR (RT PCR), single strand conformation polymorphism analysis (SSCP), immunohistochemical staining and ELISA respectively. Results ①The existence of RSV gene sequences has been detected in 17.9%(7/39) of the lung cancer tissue samples. ②Immunohistochemical analysis showed the positive reactions covered the cytoplasm in freezing sections of lung cancer tissues. ③The specific RSV bands could not be observed in the product of PCR which was short of reverse transcription in the RSV positive samples. ④Mutation of K ras gene at codon 12 has been found in 25.6% (10/39) of the cases of lung cancer by SSCP. ⑤The results of existence of RSV gene and mutation of K ras gene in lung cancer tissues were analysed statistially with Paired Enumeration Data,χ 2=0.36, P >0.05. Conclusion The results confirm the existence of RSV in lung cancer tissues, and propose that there is a relationship between RSV gene and K ras gene mutation.