肠缺血再灌流时肠内给予葡萄糖能增加肠黏膜血流量

Enteral feeding of glucose increases intestinal mucosal blood flow during intestinal ischemia/reperfusion injury

目的 探讨肠缺血再灌流损伤时肠内营养与肠黏膜血流改变的关系。 方法 SD大鼠随机分为丙氨酸组 (12只 )、葡萄糖组 (14只 )和甘露醇对照组 (10只 )。先制作空肠袋 ,将激光多谱勒探头和肠黏膜张力计放置在空肠袋两端 ,分别向袋内注入丙氨酸、葡萄糖和甘露醇。用动脉夹阻断肠系膜上动脉血流 6 0min后 ,再恢复灌流 6 0min。每 30min分别测定肠黏膜血流量和局部PCO2 张力 (PrCO2 )。 结果 缺血再灌流过程中 ,与甘露醇组比较 ,葡萄糖组肠黏膜血流量显著增加 ,PrCO2降低 ,P <0 0 1。 结论 缺血再灌流过程中 ,肠内给予葡萄糖能增加肠黏膜血流量 。

Objective To explore the relationship between enteral nutrition and the change in intestinal mucosal blood flow during intestinal ischemia/reperfusion injury. Methods Thirty six Sprague Dawley rats were randomly divided into alanine (12 rats),glucose(14 rats) and mannitol control(10 rats) groups.Jejunal sac was prepared with the filling of either 10 mM alanine,glucose or mannitol in the three groups.The laser doppler probe and intestinal mucosal tonometry were placed at the both ends of the sac.The superior mesenteric artery was occluded by arterial clamp for 60 mins and released thereafter for another 60 mins.Intestinal mucosal blood flow and regional pressure of CO 2(PrCO 2) were determined every 30 mins. Results During the process of ischemia/reperfusion,the intestinal mucosal blood flow in glucose group increased evidently and the PrCO 2 in glucose group decreased obviously(P<0 01) when comparcd with those in mannitol group. Conclusion During the process of ischemia/reperfusion,enteral feeding of glucose could increase intestinal mucosal blood flow,which provided guarding effects on the intestine suffering from ischemic/reperfusion injury. [