内毒素对人脐静脉内皮细胞形态和功能的影响

The effect of Lipopolysacharide(LPS) on morphology and function of human umbilical endothelial cells(HUVECs)

目的 研究内毒素对人脐静脉内皮细胞 (HUVEC)形态和功能的影响 ,探讨内毒素对血管内皮细胞的激活全身性炎症反应、脓毒症、多器官功能衰竭中的作用。 方法 以体外培养的人脐静脉内皮细胞为模型 ,应用倒置显微镜观察内毒素对HUVEC形态的影响 ;ELISA双抗夹心法测定IL 6的含量 ;应用免疫荧光染色法 ,激光共聚焦扫描显微镜观察细胞间粘附分子 1(ICAM 1)的表达。 结果 内毒素可明显改变HUVEC的形态 ,使其发生长梭状变形。刺激HUVEC分泌IL 6的最小剂量为 1ng/ml,呈剂量依赖性增加 ,8h达高峰。激光共聚焦扫描显微镜观察结果显示 ,LPS 10ng/ml与HUVEC作用 2 4h后 ,ICAM 1在核膜及细胞膜的表达都明显增强。 结论 内毒素可明显改变HU VEC的形态和功能 ,对增加血管通透性 ,促进白细胞粘附 ,在炎症瀑布反应的触发过程中可能起着重要的始动作用。

Objective To study the effects of LPS on the morphology and function of HUVECs,so as to explore the roles of activated vascular endothelial cells(VEC) by LPS in systemic inflammatory reaction,sepsis and MOF. Methods HUVECs primarily cultured in vitro were employed as the model.Inverted microscope was used to observe the effects of LPS on the morphology of HUVECs.ELISA was used to assess the IL 6 content.The expression of ICAM 1 was determined by immunofluorescent staining method with confocal laser scanning fluorescence microscope. Results The morphology of HUVECs could be altered by LPS to become long cord like in shape.The smallest dose of LPS for stimulating the secretion of IL 6 from HUVEC was 1 ng/ml,and the effect was enhanced parallel to the increase of the concentration of LPS and reached top level at 8 postburn hours.The expresson of ICAM 1 on the nucleic and cytoplasmic membrane of HUVECs increased obviously after HUVECs was cultured with LPS(10ng/ml) for 24 hours when observed by laser confocal scanning light microscope. Conclusion The morphology and function of HUVECs could be evidently affected by LPS.As a result,LPS might play important roles in the increase of vascular permeability,the promotion of leukocytic adherence,and the initiation of inflammatory cascade reaction. [