参麦注射液对烧伤小鼠巨噬细胞功能影响的研究

The effects of astragalus and shenmai injections on macrophage function in burned mice

目的 观察小鼠烧伤后巨噬细胞 (M)功能的动态变化 ,探讨中药黄芪和参麦注射液对烧伤小鼠M功能和存活率的影响。 方法 将小鼠按伤后时间及处理方法分为烧伤前及烧伤后2、6、12、2 4、48、72、12 0h组 ,正常对照组 (A组 )、烧伤对照组 (B组 )、正常黄芪给药组 (C组 )、正常参麦给药组 (D组 )、烧伤黄芪给药组 (E)、烧伤参麦给药组 (F)。应用吞噬法、RT PCR等方法分别测定了伤后不同时间及给予中药后烧伤小鼠M各种功能的变化。 结果 (1)伤后 12 0h内小鼠M的吞噬功能下降 ,酸性磷酸酶 (ACP)活性减低 ,IL 15mRNA表达发生波动 ,TNFαmRNA表达显著增高。 (2 )给予黄芪 (2 5 0 0mg·kg-1·d-1) 5d后 ,M吞噬功能和ACP显著升高 (P <0 0 1) ,对IL 15mRNA没有影响 ,不能降低TNFαmRNA表达 ,不能提高烧伤小鼠的存活率。 (3)给予参麦 (2 .5ml·kg-1·d-1,1ml注射液含红参 10 0mg、麦冬 10 0mg) 5d后 ,吞噬率和ACP显著升高 (P <0 0 1) ,对IL 15mRNA表达没有影响 ,但能显著降低TNFαmRNA(P <0 .0 1)表达 ,显著提高烧伤小鼠的存活率 (P <0 0 5 )。 结论 烧伤早期腹腔注射参麦注射液可明显改善伤鼠M功能 ,提高伤鼠存活率。

Objective To explore the dynamic postburn change in macrophage function in burned mice within 120 hrs after injury,and to investigate the effects of astragalus and shenmai injections on the macrophage function and surrvival rate of burned mice. Methods The mice were divided into 13 groups according to postburn time and handling methods,i,e,normal control(A),burn control (B),normal mice with astragalus(NA),normal mice with shenmai (NS),burned mice with astragal (BA),burned mice with shenmai(BS) 2 postburn hour(2 PBH),6 PBH,12 PBH,24 PBH,48 PBH,72 PBH,120 PBH groups.The changes in the various macrophage functions at different postburn time points and after the use of astragalus and shenmai injections were determined by means of phagocytic and RT PCR methods. Results (1)Within 120 PBHs,the phagocytic function of murine macrophages decreased evidently.The ACP activity decreased obviously.The expression of IL 15 mRNA fluctuated and that of TNF mRNA enhanced significantly.(2)Five days after the application of astragalus in dose of 2 500 mg·kg -1 ·d -1 ,the phagocytic function of macrophages and ACP activity increased markedly(P<0 01).The expressions of IL 15 and TNF mRNAs were not influenced.The survival rate of mice was not increased.(3) Five days after the application of shenmai injection in dose of 2.5 ml·kg -1 ·d -1 ,the phagocytic function of macrophages and ACP activity increased significantly (P<0 01),while the expression of IL 15 mRNA exhibited no change.But the expression of TNFα mRNA decreased obviously(P<0.01).Moreover,the survival rate of burned mice was evidently raised(P<0 05). Conclusion Peritoneal administration of shenmai injection at early postburn stage could significantly improve the macrophage function of burned mice,and it increase the survival rate of mice. [