Hodgkin病肿瘤细胞内存在人巨细胞病毒

Presence of Human Cytomegalovirus within the Neoplastic Cells of Hodgkin′s Disease

现已公认，Hodgkin病肿瘤组织中的H/RS细胞是肿瘤细胞，但其成因尚不清楚。10多年前，在H/RS细胞内发现存在EB病毒基因组DNA，此后许多研究均支持这一发现，表明EB病毒在Hodgkin病的发生中具有重要作用。但许多EB病毒阴性Hodgkin病的病因尚无法用EB病毒解释。笔者应用显微切割、PCR、免疫组化及原位杂交等技术，发现在H/RS细胞内存在人巨细胞病毒基因组DNA和抗原，提示人巨细胞病毒在EB病毒阴性的Hodgkin病的发病中具有重要作用。

It is generally accepted that Hodgkin(H)and Reed-Sternberg(RS)cells are the neoplastic cells in the tissue of Hodgkin′s disease． However, the etiology is still unknown． Epstein-Barr virus (EBV)genome was demonstrated in H/RS cells more than ten years ago and thereafter many studies support- ed the finding． It has been suggested that EBV plays an important role in pathogenesis of Hodgkin′s disease． However, there are many cases of Hodgkin′s disease which are EBV-negative． Thus, the search for an‘HD-associated’ virus is necessary． Using microdissection, polymerase chain reaction, in-situ hybridization and immunohistochemical methods, we found not only cytomegalovirus genome but also its antigen is localized within H/RS cells． It suggests that, in addition to EBV, human cytomegalovirus might play a role in the pathogenesis of some EBV-negative cases of Hodgkin′s disease．