双氢青蒿素、青蒿琥酯治疗大鼠肺孢子虫肺炎的疗效研究

THE THERAPEUTIC EFFECT OF DIHYDROARTEMINSININ AND SODIUM ARTESUNATE ON PNEUMOCYSTIS CARINII PNEUMONIA OF RAT MODEL

目的 研究双氢青蒿素、青蒿琥酯对实验大鼠肺孢子虫肺炎的疗效。方法 以醋酸可的松皮下注射Wistar大鼠诱导建立肺孢子虫肺炎动物模型 ,用双氢青蒿素、青蒿琥酯治疗实验大鼠 ,以蒿甲醚、复方磺胺为对照治疗组 ,通过存活率、存活天数、肺重、肺重 /体重比值、肺印片的每视野包囊均数等指标考核疗效。结果 双氢青蒿素 30mg/kg× 5d、6 0mg/kg× 5d治疗组的大鼠存活率提高 ,存活天数延长 ,治疗后体重增加 ,肺重、肺重 /体重比值、肺印片的每视野包囊均数低于模型对照组 ;青蒿琥酯 6 0mg/kg× 5d治疗组除存活率外 ,其余指标接近双氢青蒿素组 ;各治疗组的减虫率均高于 6 0 % ,接近复方磺胺组的73 .1%。结论 双氢青蒿素、青蒿琥酯对大鼠肺孢子虫肺炎具有疗效。

Aim To assess the therapeutic effect of dihydroarteminsinin (DHA) and sodium artesunate (ATS) on Pneumocystis carinii pneumonia (Pcp) of immuosuppressed experimental rats.Methods Pcp rat models were established by injection subcuteneously with cortisone acetate twice a week for 6 weeks.The Pcp rats were administered orally with DHA at dose of 60mg/kg, at 30mg/kg once a day, and injected intramuscularly with ATS at dose of 60mg/kg once a day for 5 days respectively. Therapy controls included injection intramuscularly with artemether at dose of 80mg/kg and administered orally with SMZ Co (sulfamethoxazol 250mg/kg +Trimethoprim 50mg/kg) respectively. All rats were necropsied at the end of 8th week. The evaluation of drug efficacy was decided on the base of mean cyst number per field in lung print smear, rat survival rate, survival days, increase of body weight, lung weight, lung weight/ body weight ratio.Results The lung weight, lung weight/body weight ratio, and mean cyst number per field of lung print smear of rats treated with DHA at 30mg/kg and at 60mg/kg ,and with ATS at 60mg/kg were lower than that of immunosuppressed rats control respectively. The survival rates, survival days, and the increase of body weight of rats treated with DHA at 30mg/kg and at 60mg/kg, and with ATS at 60mg/kg were more than that of the other groups respectively, which approach to that of the group treated with SMZ Co. The reduce rates of cyst in the treated groups were more than 60%, which approach to 73.1% of treatment with SMZ Co also.Conclusion The therapeutic effects of treatment of rat Pneumocystis carinii pneumonia with dihydroarteminsinin and sodium artesunate were active.