日本血吸虫病经胎盘传播免疫耐受性的进一步研究

THE FURTHER RESEARCH OF IMMUNOLOGIC TOLERANCE OF CONGENITAL TRANSMISSION OF SCHISTOSOMIASIS

目的 进一步探索日本血吸虫病经胎盘传播的免疫耐受性。方法 对不同孕期的怀孕母兔分别感染 30 0条日本血吸虫尾蚴 ,感染 45天后收集母兔和仔兔血清 84份 ,在检测家兔日本血吸虫特异性IgG抗体研究结果的基础上 ,进一步以ELISA方法检测日本血吸虫特异性IgM抗体。结果 10份母兔血清IgM抗体检测均为阳性 (OD =0 .82～ 1.5 1) ,74份仔兔血清均为阴性 (OD =0 .0 1～ 0 .49) ;怀孕中期感染日本血吸虫母兔所产仔兔 (5 2份 )和怀孕晚期感染所产仔兔 (2 2份 )血清 ,OD均值分别为 0 .2 3和 0 .2 8,两者差异有显著意义 (P <0 .0 5 ) ;同一怀孕期感染日本血吸虫母兔所产仔兔血清 ,OD值差异无显著意义 (P >0 .0 5 )。结论 日本血吸虫经胎盘传播后 。

Aim To explore the immunologic tolerance in congenital transmission of Schistosoma japonicum.Methods Each mother rabbits in different gestation period was infected 300 cercaria of S.japonicum.84 sera of mother rabbits and kittens were collected 45 days after infection.Based on the detection of special anti-schistosma IgG antibody of the rabbits,IgM were further detected by ELISA.Results 10 sera of mother rabbits were positive (OD value between 0.82～1.51),while 74 sera of the kittens all were negative (OD value between 0.01～to 0.49).The sera of the kittens from mother rabbits infected in mid gestation and late-gestation were detected,and the OD value were 0.23 and 0.28,respectively.That showed significant different between two group.(P<0.05).In two groups,the sera of kittens of recovered and un-recovered worms were also detected by ELISA.It showed no significant differs(P>0.05).Conclusion The kittens of congenital transmission of S.japonicum exist immunologic tolerance.