酵母全基因组中新的ORF结构的预测

Predicting New ORFs in Yeast Genome

提出了一个预测 DNA序列中无内含子的开阅读框架 ( ORF)的理论方法—终止密码预测法 ;用酵母全基因组中已知的 62 60个基因进行检验 ,预测成功率为 99.9% ;发现酵母基因编码区和已知 DRF的起始密码子总是位于长距离不出现终止密码的位置序列上游紧邻最后一个终止密码子的 ATG;在酵母全基因组 DNA中发现了新的长度不小于 90个氨基酸的ORF结构 2 2 4

A method for predicting first kind of open reading frame (ORF) is given,which is called Terminal Codon Method.The first kind of ORFs is the ORF that has not intron in it.The correctness of the method is examined by 6 260 known genes and ORFS of yeast genome and its successful rate is 99.9%.This result shows that the starting codon of yeast gene is always the ATG which is in close neighbor to the last terminal codon upstream.2 244 new ORFs with length not lower than 90a.a have been found.