酪蛋白酶解制备酪蛋白磷酸肽研究 Ⅱ.产物分析及反应动力学模型

Enzymatic hydrolysis of casein to phospopeptides Ⅱ.Hydrolysate analysis and kinetic model

利用高效液相排阻色谱对酪蛋白──碱性蛋白酶水解产物分子量分布进行了分析。在最适酶解工艺条件下，求得各表观动力学常数：Km=0.3132g/L,Kcat=12.9248/min，Ks=9.5565g/L,Kp=0.00285g/L。建立了考虑酶一级热失活、底物反竞争性抑制和产物竞争性抑制三方面因素共同影响的本征反应动力学模型，经实验拟合平均相对误差为6.21％。

Molecular weight distribution of casein－alcalase hydrolysates was analyzed by HPSEC.Under the optimal operating conditions,the kinetic constants were experimentally determined as Km=0.3132g/L,Kcat=12.9248/min，Ks=9.5565g/L,Kp=0.00285g/L.Taking into account the substrate inhibition,product inhibition and enzyme inactivation,a simplified kinetic model was established.The regressive results indicated that the average relative error was 6.21％.