化学杂交剂对油菜胞质不育系波利马育性的影响

EFFECTS OF A CHEMICAL HYBRIDIZATION AGENT ON THE FERTILITY OF POLIMA MALE STERILE LINES OF RAPE ( BRASSICA NAPUS L.)

用杀雄剂２号在造胞细胞阶段、花粉母细胞减数分裂期和单核期处理波利马细胞质不育系油菜Ｌ１７Ａ。结果表明，以０．００７５％和０．０１％的杀雄剂在花粉母细胞减数分裂期和单核期处理，初花期无花粉型不育株的比例和天然异交率明显提高，自交结实率、结果率、单株粒数和单株有效果数显著降低，但对制种产量无明显影响。说明杀雄剂２号能在花粉母细胞减数分裂期和单核期阻断微量花粉的正常发育，减少甚至消除微量花粉。

In order to solve the problem of trace pollen on Polima cytoplasmic male sterility (Pol CMS), male-gametocide No. 2 (MG 2) of different concentrations were spplied to L17A, one of Pol CMS lines of rape (Brassica napus) at the sporogenic stage, meiosis stage and uninucleate cell stage. The results of two-year experiment showed that the meiosis stage and uninucleate stage were most sensitive and 0.0075%～0.01% MG 2 significantly decreased self cross percentage, pod setting percentage, self cross seed number per plant and pot setting per plant, and greatly increased the percentage of non-pollen flower plants and natural cross. Thus, it was proved that MG 2 used at the meiosis stage and uninucleate stage of the pollen mother cell can stop the normal development of trace pollen in Pol CMS and consequently reduce and even eliminate trace pollen.