摘要
用杀雄剂2号在造胞细胞阶段、花粉母细胞减数分裂期和单核期处理波利马细胞质不育系油菜L17A。结果表明,以0.0075%和0.01%的杀雄剂在花粉母细胞减数分裂期和单核期处理,初花期无花粉型不育株的比例和天然异交率明显提高,自交结实率、结果率、单株粒数和单株有效果数显著降低,但对制种产量无明显影响。说明杀雄剂2号能在花粉母细胞减数分裂期和单核期阻断微量花粉的正常发育,减少甚至消除微量花粉。
In order to solve the problem of trace pollen on Polima cytoplasmic male sterility (Pol CMS), male-gametocide No. 2 (MG 2) of different concentrations were spplied to L17A, one of Pol CMS lines of rape (Brassica napus) at the sporogenic stage, meiosis stage and uninucleate cell stage. The results of two-year experiment showed that the meiosis stage and uninucleate stage were most sensitive and 0.0075%~0.01% MG 2 significantly decreased self cross percentage, pod setting percentage, self cross seed number per plant and pot setting per plant, and greatly increased the percentage of non-pollen flower plants and natural cross. Thus, it was proved that MG 2 used at the meiosis stage and uninucleate stage of the pollen mother cell can stop the normal development of trace pollen in Pol CMS and consequently reduce and even eliminate trace pollen.
出处
《西南农业大学学报(自然科学版)》
CSCD
1999年第2期140-143,共4页
Journal of Southwest Agricultural University
基金
四川省"九五"育种攻关
重庆市应用基础研究项目
关键词
甘蓝型油菜
波利马细胞质雄性不育系
化学杀雄剂
育性
Brassica nopus L
cytoplasmic male sterility
pollen
chemical hybridization agent (CHA)