摘要
研究了醇类和丙酮、二甲亚砜等水溶液极性对Ｈ２Ｏ２酶活力的影响。结果表明，Ｈ２Ｏ２酶的活力随着溶液极性的降低按线性规律下降。在丙醇、氯乙醇水溶液中，Ｈ２Ｏ２酶具有稳定的中间态，当二者浓度极低时，Ｈ２Ｏ２酶可被激活，活力最大可提高２０％和２６％。溶液极性对Ｈ２Ｏ２酶活力的影响可逆。有机溶剂使Ｈ２Ｏ２酶的吸收光谱峰值从２７０ｎｍ移到２８０ｎｍ。
In a study of alcohols, acetone and dimethyl sulfoxide, catalase activity decreased linearly as their water polarity decreased. In 1-propanol and chloroethanol water solution catalase had stable intermediate states, and when their concentrations were low, catalase was activated and its activity increased by as much as 20% and 26%, respectively. The effect of solution polarity was revertible. Organic solvents shifted the peak of catalase absorption spectrum from 270 nm to 280 nm.
出处
《西南农业大学学报（自然科学版）》
CSCD
1999年第2期186-189,共4页
Journal of Southwest Agricultural University
