不同种群小菜蛾对4种杀虫药剂的抗性及抗性基因频率

Insecticide Resistance and the Genotype Frequencies of Genes in the Diamondback Moth (Plulella xylostella L.) from Different Populations

用 FAO推荐的点滴法测定了日本、台湾、广州、东莞等地小菜蛾 (Plutella xy-lostella L .)的抗药性。测定结果表明 :日本、广州的小菜蛾对阿维菌素显示出抗药性 ,其抗性比率分别是 4 .85倍和 10 .2 7倍。杀虫药剂抗性基因型频率测定显示 ,广州、广东东莞和北京种群的最高 ,基因型频率分别为 4 1.2 5%、4 1.2 5%和 58.2 3%。研究验证了快速测定杀虫药剂抗性基因型频率的方法 ,并提出了根据不同种群小菜蛾抗性基因型频率所应采取的综合治理策略。

The resistance of the diamondback moth (DBM) to bissultap, dichlorvos, malathion and abamectin was tested using the typical method of FAO. DBMs from Beijing, Guangzhou, Japan as well as strain from Taiwan were sampled. The results showed that the DBMs collected from Japan, Guangzhou are very strongly resistant to dichlorvos. The resistant rates of abamectin to Japan and Guangzhou populations are 4.85 and 11.29 times respectively. The highest resistant genotype frequencies are formed in Guangzhou, Dongwan, and Beijing DBM populations, with the resistant genotype frequencies of 41.25%, 41.25% and 58.23% respectively. A quick method of determining insecticide resistance genotype frequencies was verified, and the tactics of integrated managements according to the insecticide resistance genotype frequencies of the DBMs in different populations was discussed.