反相高效液相色谱用于寄生虫中氨基酸含量的测定

Reversed-Phase High Performance Liquid Chromatography Used for Determination of Amino Acids of Parasites

为了对寄生虫病进行有效的防治 ,采用柱前衍生、反相高效液相色谱分离、紫外吸收定量法对中国 7个地区马来丝虫及马来丝虫幼虫氨基酸的组成和含量进行了测定。结果表明 :不同地区的马来丝虫中各氨基酸的含量存在着一定的差别 ,幼虫的氨基酸含量明显低于成虫的相应氨基酸含量。此方法简便、准确 ,在研究寄生虫的种内分化、种下分类和生长、发育。

The compositions and contents of amino acids of parasites in different areas of China have been determined by precolumn derivatization, reversed phase high performance liquid chromatography (HPLC) separation and ultra violet absorption quantitation in order to prevent diseases caused from parasites. The results show that there were some differences in contents of amino acids in B.malayi from seven different areas in China. The contents of amino acids gradually increased from the southern to the northern areas in China. The method is simple and accurate, and the results have important values to investigate the life activities of parasites.