维甲酸和维甲类X受体各亚型基因在原发性肝癌组织中表达

THE EXPRESSION OF RETINOID ACID RECEPTORS AND RETINOID X RECEPTORS IN HEPATOCELLULAR CARCINOMA AND SUROUNDING CIRRHOTIC TISSUES

目的 维甲类药物的分化诱导作用受特定组织表达受体的影响 ,了解肝癌组织表达维甲酸受体和维甲类X受体的表达 ,以便选择特异的维甲类药物治疗肝癌。方法 根据GeneBank的序列 ,设计对RARα、RARβ、RARγ、RXRα、RXRβ、RXRγ基因的 6对引物 ,用RT PCR的方法检测 18例肝癌组织和相应癌周肝硬化组织这些基因的表达。结果 这些基因在肝癌组织表达率分别为 44 4% (8/18) ,16 7%(3/18) ,77 8% (14/18% ) ,88 9% (16 /18) ,77 8% (14/18) ,16 7% (3/18) ;癌周肝硬化组织分别为 2 7 8% (5 /18) ,0 % (0 /18) ,6 1 1% (11/18) ,77 8% (14/18) ,5 5 6 % (10 /18) ,0 % (0 /18)。这些受体亚型表达的阳性率在肝癌组织似高于癌周肝硬化组织 ,但统计学无显著意义 (P >0 0 5 )。以 β actin为内标测定RARs和RXRs的相对表达量 ,RXRβ相对表达量高于癌周肝硬化组织 (配对秩和检验 ,P =0 0 2 0 8)。结论 由于肝癌组织表达有RARs和RXRs,提示除了应用全反式维甲酸治疗肝癌外 ,尚探讨RXRs选择性维甲类药物治疗价值。

Objective To study the expression of RARs and RXRs subtypes in hepatocellular carcinoma (HCC) and to facilitate the use of receptor selected retinoids in HCC patients. Methods The primers of PCR were designed based on the sequence of gene bank and the expression of RARs and RXRs were detected with RT PCR in 18 HCC tissues and the coresponding cirrhotic tissues.Results The expression of RARα,RARβ,RARγ,RXRα,RXRβ and RXRγ were 44.4%(8/18),16.7%(3/18),77.8%(14/18),88.9%(16/18),77.8%(14/18) and 16.7(3/18) in HCC tissues respectively, and were 27.8%(5/18),0%(0/18),61.1%(11/18),77.8%(14/18),55.6%(10/18) and 0%(0/18) in surounding cirrhotic tissures respectively. The positive rate of RARs and RXRs seemed higher in HCC tissues than in cirrhotic tissues, but the difference is not significant ( P >0.05). Relative expression extents of RARs and RXRs control to actin were compared between HCC and cirrhotic tissues, RXRβ expression was higher in HCC than in cirrhotic tissues ( P =0 0208).Conclusion All the subtypes of RARs and RXRs are detecte with RT PCR in HCC tissue, while the main subtypes are RARγ,RXRα and RXRβ. Therefore the therapeutic value of the selsction of RXRs retinoids in the treatment of HCC shall be further studied.$$$$