喉癌及癌前病变组织端粒酶活性检测

DETECTION OF TELOMERASE ACTIVITY IN LARYNGEAL CARCINOMA AND PREMALIGNANT LESIONS

目的 探讨喉鳞状细胞癌及喉癌前病变组织的端粒酶活性表达及其在喉癌发生中的作用。方法 采用多聚酶链反应 酶联免疫吸附法 (PCR ELISA)对 47例喉鳞状细胞癌组织、2 2例喉癌前病变组织、2 0例喉炎性息肉组织的端粒酶活性进行定量检测。结果 47例喉鳞癌组织中 39例端粒酶呈阳性 (83 % ) ,而 2 0例喉炎性息肉组织端粒酶均为阴性 ,两组具有显著性差异 (P <0 0 0 1)。 2 2例喉癌前病变组织中有 15例端粒酶表达阳性 (6 8 2 % )。结论 端粒酶在喉癌组织中有较高的表达 ,可作为喉癌的肿瘤标志物。在喉癌前病变组织中也有低水平的端粒酶活性表达。喉癌前病变组织端粒酶活性检测将有助于喉癌的早期发现和早期治疗。

Objective To investigate the telomerase activity in human laryngeal squamous cell carcinoma (LSCC) and laryngeal premalignant lesions, and to evaluate the role of telomerase activity in the genesis of LSCC.Methods PCR ELISA method was used to determine the telomerase activity in 47 specimens of LSCC, 22 specimens of laryngeal premalignant lesions and 20 specimens of laryngeal inflammatory polyps. Results Telomerase activity was detected in 39 of 47 cases of LSCC (83%). but was not detected in all inflammatory polyps. The difference was significant ( P <0.01). The detection rate of 22 biopsies of premalignant lesions was 68.2%. Conclusion Telomerase is sctivated frequently in LSCC and may be used as a biomarker. It is also activated in laryngeal premalignanat lesions. Therefore, the detection of telomerase activity will be helpful in the early diagnosis and treatment of laryngeal malignant lesions.$$$$