长江流域南方鲇(Silurus meridionalis)和鲇(Silurus asotus)分子遗传标记的建立

Establishment of the molecular genetic marker for identifing Silurus mer idionalis and Silurus asotus in Yangtze River

目的 为南方鲇和鲇的分类提供分子鉴定的依据。方法 随机扩增多态性DNA技术 (RAPD)。结果 建立了南方鲇和鲇的分子遗传标记 ,即S4 4 9 1.0kb。结论 南方鲇和鲇的分子遗传标记可在遗传育种中用于物种的有效鉴定 ,应用随机扩增多态性DNA技术可以发现并建立两个种之间鉴别的分子遗传标记。

Objective To provide basis for strain iden ti fing Silurus meridionalis and Silurus asotus. Methods Randomly amplified polymorphic DN A(RAPD) Results Established the molecular genetic marker. Conclutions The molecular genetic marker of strains can be es tablished by RAPD and the genetic marker of S449 1.0kb can be used for identifi ng Silurus meridionalis and Silurus asotus in breeding.