摘要
用副鸡嗜血杆菌Hpg_8菌株制备ELISA抗原 ,与抗鸡Ig单抗 1B7酶结合物建立了检测鸡血清抗体水平的间接ELISA方法。交叉试验、阻断试验、重复性试验等表明该方法重复性好、特异性强、灵敏度高。确立了检测ELISA效价 (ET)的回归方程y =1 4 2 1+ 0 2 99x) (P >0 0 5 ) ,可用于定量测定。间接凝集试验与间接ELISA方法的比较试验表明 ,ELISA法比间接凝集试验敏感性高 3倍以上。
The indirect ELISA method for detecting antibody of Haemophilus paragallinarum (H.P) was developed by using supersonicated H.P as coating antigen and enzyme conjugate of mouse against light chain of chicken Ig. The method has good repeativity, high specificity, and sensitivity. A detecting titer of coefficient relation y=1.421+0.299(P>0.05) was developed under 100_fold diluted sera sample. The ELISA is 3 times more sensitive than indirect hemagglutinatio test(IHT).
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2001年第6期454-457,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
天津市青年科学基金 ( 983 70 1711)资助项目
