H9亚型禽流感油乳剂灭活苗免疫产生期内T淋巴细胞表型亚类的检测与研究

Monitoring of T Lymphocytes in SPF Chickens following Vaccination with H9 Subtype Inactivated Oil_Emulsion Vaccines against Avian Influenza

采用免疫荧光法、使用流式细胞检测仪对经H9亚型禽流感病毒人工感染SPF鸡、H9亚型禽流感油乳剂灭活苗免疫SPF鸡以及经免疫后使用H9亚型禽流感病毒攻毒后的SPF鸡外周血、脾脏、胸腺中T细胞表型亚类 (CD4 +、CD8+、TCR1+)的变化规律进行了监测 ,结果表明 ,H9亚型禽流感油乳剂灭活苗免疫后抗原的缓慢释放可在一定程度上激发机体的细胞免疫应答 ,使免疫活性T淋巴细胞得到活化 ,免疫后鸡体外周血中CD4 +、CD8+和TCR1+T细胞的数量呈现出一明显升高的过程 ;同时 ,人工感染免疫鸡后 ,脾脏和胸腺TCR1+T细胞的数量上升 ,外周血CD4 +、CD8+和TCR1+T细胞的数量少量降低或维持不变 ,随后短期即恢复正常 ;而人工感染SPF对照鸡后 ,外周血CD4 +。

In this study,T lymphocytes subtypes in peripheral blood,thymus and spleen of SPF chickens vaccinated by H9 subtype inactivated oil_emulsion vaccines against avian influenza,SPF chickens challenged by avian influenza virus and vaccinated SPF chickens challenged by H9 subtype avian influenza virus were evaluated by IFA test.The results indicated that vaccination of H9 subtype inactivated oil_emulsion vaccines against avian influenza stimulated effectively cell_mediated immune response of chickens and activated T lymphocytes,The numbers of CD4 +,CD8 +,TCR +_expressing cells in peripheral blood were increased after vaccinated with inactivated oil_emulsion vaccines.After chickens vaccinated with inactivated oil_emulsion vaccines were challenged by H9 AIV,the numbers of TCR +_expressing cells were increased in thymus and spleen,the numbers of CD4 +,CD8 +,TCR +_expressing cells in peripheral blood were decreased a little or did not change,but decreased sharp in all control and in SPF chickens challenged by H9 AIV.