普陀水仙优良新品种组培繁殖技术研究

Study on the Propagation Technique in Vitro for New Varieties of Narcissus tazetta var. chinensis Rome

普陀水仙(Narcissus tazetta var.chinensis Rome)属石蒜科水仙属的多年生球根花卉。盛产于我国舟山群岛。其花有单瓣和重瓣之分,单瓣的称“金盏银台”,重瓣的称“王玲珑”。它们以苍翠碧绿、风韵清雅之叶,绚丽多姿、别致幽香之花,深为古今中外花卉爱好者所欣赏。1979年,舟山市林科所在人工栽培过程中,从1.8万株水仙中发现了有开纯白花的和开鲜黄花的,分别被命名为“白玉水仙”和“金口水仙”。它们的花型与普通单瓣品种相同,均为单瓣花,花瓣6片如盘,副花冠杯状。白玉水仙花瓣和副花冠均为白色,香气淡雅。

In this paper,the propagation technique in vitro for the new varieties of Narcissus selected from N. tazetta var. chinensis Rome was reported. In order to conserve germplasm resources of Narcissus and achieve the level of mass production, we conducted a series of synthetical systematic experiments for 3 years. The result showed that the bulbils were induced on twin scales on MS medium containing 0~5 mg/L BA, 0~1 mg/L NAA and a little activated-carbon. The percentage of induction reached 70%. More bulblets could be obtained through subculture in the same medium. If the bulbils were transplanted in 1/2MS medium with 0.1mg/L IBA in shade-culture, the rooting rate could be raised in the dark to 79.3 %. If in vitro bulbils were cultivated in the field they might flower after 3 years.