松毛虫质型多角体病毒杀虫剂杂菌含量检测及控制方法研究

Detections on the Other Microbial Contaminants in the CPV Insecticide for Pine Caterpillar and Studies on the Control Methods

对松毛虫质型多角体病毒杀虫剂中的杂菌检测表明,制剂中不含高等动物致病茵,细菌不再继续增殖。抑灭菌试验表明,适当浓度的洗必泰、洁尔灭、磺胺及苯甲酸钾等对制剂中的真菌有控制效果。用丙酮按1:1处理离心沉淀物使制剂中的杂菌含量减少了1000倍。用等量过饱和乳糖丙酮处理离心沉淀物可获得粉剂。经测定,除叠氮钠处理的制剂外,其它处理在毒力上与对照无差异。

The results of detections on the microbial contaminants in CPV agent for Pine Caterpillar (Dendrolimus spp.) showed that there were not enteric pathogenic microorganisms for human beings in the agent, the germ in the agent was not generative and the fungus in the agent could be controlled by inhibitors and disinfectants practiced in the test. The microbial contaminants in the agent were decreased by a thousand times when the pellet of the agent was treated with acetone,volume/wet-weight =1:1.A powder was easily obtained through treating the pellet of the agent with supersaturated-lactose-acetone in the ratio of 1:1. The results of bioassay showed that the treated and untreated agents did not indicate any evident difference in pathogenicity except the sample treated with sodium azide.