人白细胞L－myc遗传多态性分析

Analysis of L-myc polymorphic Patterns in Human White Blood Cell

从人白细胞中分离ＤＮＡ，经ＥｃｏＲＩ酶切后，进行Ｌ－ｍｙｃＳｏｕｔｈｅｒｎ杂交，得出人Ｌ－ｍｙｃ限制性内切酶片段长度多态性（ＲＦＬＰ）图谱。结果发现，经ＥｃｏＲＩ酶切产生Ｌ－带（１０ｋｂ）及Ｓ－带（６ｋｂ）。对Ｌ－ｍｙｃＲＦＬＰ与肺癌恶变与预后的关系进行了讨论。

A differences of L-myc restriction-fragment-length polymorphism(RELP) of DNA from human white blood cell have been determined, by Southern blot hybridazation.It was shown L-band(10kb)and S-band(6kb) produced by EcoRI digestion. The ralationship between L─myc RFLP and prognosis and matastasis in lung cancer patients were discussed.