不同背景抗－HBs，抗－HBc血清中HBVDNA的PCR研究

Study on HBVDNA of Different Background anti-HBs and anti-HBc Serum Samples by PCR

为探讨ＨＢＶ感染后不同背景抗－ＨＢｓ，抗－ＨＢｃ的意义，用多聚酶链式反应（ＰＣＲ）检测ＨＢＶ感染者血清中ＨＢＶＤＮＡ。抗－ＨＢｓ（＋）病例的ＨＢＶＤＮＡ检出率显著性低于抗－ＨＢｓ（－）病例的检出率，支持抗－ＨＢｓ对机体的保护作用；１７．７％单项抗－ＨＢｓ（＋）的既往感染者有ＨＢＶＤＮＡ的存在，说明抗－ＨＢｓ对ＨＢＶ的清除受其它因素的影响；单项抗－ＨＢｃ，抗－ＨＢｃ／抗－ＨＢｓ，抗－ＨＢｃ／ＨＢｓＡｇ，ＨＢｅＡｇ阳性的病例有不同的ＨＢＶＤＮＡ检出率，说明不同血清学背景的抗－ＨＢｃ有不同的ＨＢＶ复制情况。不同类型的肝炎ＨＢＶＤＮＡ检出率无显著性差异。

In order to evaluate the significance of anti-HBs and anti-HBc,HBVDNAs of different background anti-HBs(+)and anti-HBc(+)serum samples were tested by polymerase chain reaction.Results:The positive rate of anti-HBc(-)was higher than that of anti-HBs(+).This suggests that anti-HBs is a“protector”.HBVDNAs were found in 17.7%only anti-HBs(+).The result shows that the effect of anti-HBs are influenced by some unknown factors.Differet positive rates were found in anti-HBc(+)samples with different serum markers background. No statistically significant correlations were found between HBVDNAs of chronic and acut B hepatitis.